Journal: Translational Lung Cancer Research

Article Title: Osimertinib resistance-based immune prognostic related gene signature in EGFR mutant lung adenocarcinoma, in which PSMD11 promotes tumor progression

doi: 10.21037/tlcr-2025-355

Figure Lengend Snippet: Knockdown of PSMD11 could enhance the antitumor effects of OSI in vivo . (A-C) Subcutaneous transplantation tumor pictures, tumor growth curves, and body weight plot after subcutaneous injection with control or PSMD11 knockdown PC9OR cells (n=5). (D-F) Subcutaneous transplantation tumor pictures, tumor growth curves, and body weight plot after subcutaneous injection with control or PSMD11 knockdown H1975OR cells (n=5). (G-I) Immunohistochemistry showed representative pictures of Ki-67 in different treatment groups of PC9OR and H1975OR cells, and the positive expression rate was displayed using a bar plot. (J) Immunohistochemistry showed representative images of cleaved-caspase3 and TUNEL in different treatment groups of PC9OR and H1975OR cells. (K) The protein expression of apoptosis-related molecules in tumor samples of PC9OR and H1975OR cell xenograft models after surgery in different treatment groups was detected by western blotting. *, P<0.05 compared with control group; **, P<0.01 compared with control group; ***, P<0.001 compared with control group; ## , P<0.01 compared with OSI group; ### , P<0.001 compared with OSI group; △△ , P<0.01 compared with PSMD11-siRNA group. NC, negative control; OSI, osimertinib; siRNA, small interfering RNA; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.

Article Snippet: The dilution ratios of primary antibodies, rabbit anti-PSMD11 (14786-1-AP, Proteintech) and mouse anti-caspase3 (66470-2-Ig, Proteintech), were 1:200.

Techniques: Knockdown, In Vivo, Transplantation Assay, Injection, Control, Immunohistochemistry, Expressing, TUNEL Assay, Western Blot, Negative Control, Small Interfering RNA, End Labeling

Journal: Biomaterials Research

Article Title: Custom-Made Ce–Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization

doi: 10.34133/bmr.0118

Figure Lengend Snippet: CeMn-PEG ameliorated H 2 O 2 -induced inflammation and ECM degradation and reduced H 2 O 2 -induced apoptosis and autophagy in vitro. (A) The relative mRNA expression of IL-1β, IL-4, IL-6, IL-10, collagen II, aggrecan, MMP13, and ADAMTS5 was determined by using qPCR. (B and C) Representative images and quantification data of Western blot results of IL-1β, IL-4, IL-6, IL-10, collagen II, aggrecan, MMP13, and ADAMTS5. (D and E) Representative images and relative fluorescence intensity of double immunofluorescence of LC3 (red) and C-caspase3 (green) in NP cells. (F) Representative images and quantification data of Western blot results of LC3, p62, and C-caspase3. Scale bar, 25 μm. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3.

Article Snippet: Subsequently, samples were incubated with primary antibodies against CD86 (Bioss, #bs-1035R, 1:300), CD206 (Affinity, #DF4149, 1:300), C-caspase3 (Affinity, #AF7022, 1:300), and LC3 (CST, #83506S, 1:300) overnight at 4 °C.

Techniques: In Vitro, Expressing, Western Blot, Fluorescence, Immunofluorescence

Journal: Biomaterials Research

Article Title: Custom-Made Ce–Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization

doi: 10.34133/bmr.0118

Figure Lengend Snippet: RAW264.7 cells pretreated with CeMn-PEG could alleviate H 2 O 2 -induced ECM degradation and apoptosis in NP cells. (A) Diagram of RAW264.7 cells (apical chamber) and NP cells (basal chamber) cocultured by using Transwell dish. RAW264.7 cells were pretreated with CeMn-PEG and then cocultured with NP cells. (B and C) Representative images and quantification data of Western blot results of collagen II, aggrecan, MMP13, and ADAMTS5 in NP cells in the coculture system. (D) Representative images of flow cytometry analysis of NP cells in the coculture system. (E) Apoptosis rate of NP cells in the coculture system estimated by flow cytometry analysis. (F and G) Representative images and relative fluorescence intensity of C-caspase3 (green) in NP cells in the coculture system. Scale bar, 25 μm. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3.

Article Snippet: Subsequently, samples were incubated with primary antibodies against CD86 (Bioss, #bs-1035R, 1:300), CD206 (Affinity, #DF4149, 1:300), C-caspase3 (Affinity, #AF7022, 1:300), and LC3 (CST, #83506S, 1:300) overnight at 4 °C.

Techniques: Western Blot, Flow Cytometry, Fluorescence